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the effect of spectral light quality on in vitro culture of sugarcane/ efeito da qualidade espectral da luz no cultivo in vitro de cana-de-acucar.

by:Dolight LED Panel     2019-09-01
Introduction of sugarcane (Saccharum spp. , Poaceae)
It is an important agricultural crop that can produce valuable by-products.
Products such as sugar, bio-fuel, bio-fiber, wax, bio-plastic (
Singh, Kumar, tivali, lastoji, Singh, 2013).
Brazil is the world\'s leading producer of sugar cane, followed by India and China (
Montero and Santa Clara, 2014).
Among several commercially grown sugarcane varieties developed by RIDESA (
University Network for sugarcane industry development in Brazil)
Breeding Program RB867515 is one of the most frequently planted varieties in Brazil, with drought resistance, high sucrose content, rapid growth and high productivity (
Daros, Oliveira, Zambon, and bespalock Filho, 2010).
In vitro reproduction of sugarcane has received considerable attention recently due to its economic importance.
Micro-breeding technology promotes the rapid reproduction of newly developed varieties and ensures high quality and the production of diseasesFree seedlings (
Snyman, red wine, Koch Banasiak, WA, 2011).
Although sugar cane is one of the major breeding species in the Brazilian plant biological plant (
Gerald, Lee, 2011)
There are some restrictions on the feasibility of sugar micro-breeding.
In fact, high production costs are a very important issue, so new technologies need to be developed.
Innovation, such as the use of LEDs (LEDs)
Instead of white fluorescent lamps, satisfactory results have been produced for various In vitro cultured plants such as cotton, anzuhua, vegetable seedlings and bananas (Budiarto, 2010;
Li, Xu, Tang, 2010;
Li, Tang, Xu, 2013; Vieira et al. , 2015).
Compared with the traditional lighting system, LED lighting system has several advantages.
These include low energy consumption, longer bulb life and maximum PAR efficiency between 80 and 100%, while their fluorescent lights provide only 20-30% (
Dako, haidalizad, shawfs and sabaryan, 2014).
Due to the high luminous efficiency, LED bulbs produce less heat, thus indirectly reducing the cost of refrigeration.
Another advantage that Led provides is the versatility of the controlled spectral components required by plants for various morphological reactions (
Gupta & Jatothu, 2013; Darko et al. ,2014).
Since different spectral light quality affects the morphological and/or physiological aspects of plants under in vitro culture conditions, the effects of changes in the light spectral profile on sugarcane growth and reproduction were evaluated.
Materials and methods for plant materials and growth conditions of sugarcane plants (
Previously established in vitro, produced by Biofactory Governador Arraes (Recife,Brazil)
Transfer to the test tube with a MS of 10 ml (
Murashige & Skoog, 1962)
Liquid medium with 3% sucrose, 100 mg [added]L. sup. -1]myo-inositol and 1. 3 [micro]M BAP.
The pH value of the medium was adjusted to 5.
8 before sterilization, maintain the culture within 24 days in the growth room of 25 [+ or -]2[degrees]
C, 16 light, one of the five light treatments.
There are two parts of each light-
LEDs set: a light-emitting circuit that is easy to disassemble and a direct current supply for controlling the luminous intensity through current regulation.
Lighting system consisting of rgb led-LEDbars (IHS, NewYork)
Each contains 15 TripleLED-chips. LED-
Assemble and secure the bar in the panel (40 X 30 cm)
Placed about 10 cm above the tube.
Experimental plants are randomly assigned to each light treatment and its corresponding Photosynthetic Photon flux (PPF)
The details are as follows :(1)
B: R = 70: 30, 70% blue light, 30% red light (72[micro]mol [m. sup. -2][s. sup. -1]); (2)
B: R = 50: 50, 50% blue light and 50% red light (60 [micro]mol [m. sup. -2][s. sup. -1]); (3)
B: R = 30: 70, 30% blue light, 70% red light (53 [micro]mol [m. sup. -2][s. sup. -1]); (4)WL= 100% white-LED (77 [micro]mol [m. sup. -2][s. sup. -1])and (5)
FL = fluorescent lamp (46 [micro]mol [m. sup. -2][s. sup. -1]).
Typical spectrum from LED
The Marine optical model USB2000 of the optical fiber integrated spectrometer records the bar system and the fluorescent lamp (Figure 1).
Seedling Growth and reproduction evaluate the effect of each light treatment according to the following growth parameters: stem length, fresh mass, number of till and number of leaves per plant.
The length of the stem from the bottom of the stem to the last expanded leaf was measured with Argos.
To weigh fresh substances, remove the material from the culture tube and weigh it immediately with an analytical balance to prevent dehydration.
Number of leaves (
Green and aging)
The seedlings were observed and counted.
The content of photosynthetic pigments extracted was chlorophyll and carrot-like 0.
Use 2 g fresh leaf tissue and grind the sample with mortar.
With 20 ml (V)
Acetone 80%, light density measured by UV
Visible Spectrometer (SP-
St. Paul, Biospectro, 220)
On chlorophyll a at 663 nm (Chl-a)
, On the chlorophyll B at 645 nm (Chl-b)
Carrots are at 470 nm.
Equation for Lichtenthaler weredetermined content of chlorophyll and carotene (1987).
The experiment was designed with complete random design, 5 light treatments and 25 repetitions.
The experimental device consists of a test tube containing a plant.
The data is processed by ANOVA and the average difference is by Scott-
Use the software assistant ver for notttest. 7. 6 beta (
Campcg, campinagland).
Results The effects of different light spectral quality on growth and multiplication the light treatment promoted different responses to growth parameters (Table 1).
Compared to other treatments, the longest stems appeared in plants cultivated under B: R = 50: 50 led lights.
B: R = 70: 30 and 50: 50 LED combinations and the highest total fresh quality appears in fl.
In terms of the total number of leaves, FL showed the highest average compared to other leaves.
The average number of green leaves is higher in B: R = 70: 30, B: R = 50: 50 and FL, while leaf aging is favored by B: RLEDs, 50: ratio of 50 and 30: 70 and FL.
In LED lights B: R = 30: 70, the number of tillnumber er, there is no statistical difference in other treatments.
However, seedlings grown under fluorescence spectrum present the highest proliferation rate digitally (1:3. 33)
B: R = 50: 50 LED lights (1:3).
The effect of light spectral quality on the pigment content, chlorophyll and carrot-like concentration of sugarcane leaves varies depending on the spectral optical quality (Figure2). Chl-
The a content in WL is the highest, followed by blue/red led processing.
Minimum concentration of chlorophyll-a, Chl-b and Chl (a+b)
Found in plants cultured in FL and B: R = 70: 30 LED lights. Chl-fc andChl (a+b)
There was no statistically significant difference in the content in other spectra studied.
Exposure to WL is conducive to the accumulation of carrot-like pigments.
Discuss that the growth and development of plants are strongly influenced by the quality of the spectral light, or rather, the wavelength at which it reaches the surface of the plant (Chen et al. , 2014)
Several anatomical, physiological, morphological and biochemical parameters were affected.
Current results indicate that the growth parameters evaluated are affected by the applied light treatment (Table 1).
In LED light treatment, B: R = 50: 50 promoted the highest stem length, fresh yield and tillering of sugarcane plants.
Similar results of blue/red LED with the same proportion were obtained in cotton (Li et al. , 2010)
RB92579 in the sugar industry (Silva et al. , 2014).
The mixture of blue and red light sources can compound the advantages of one-color red and one-color blue light (Li et al. , 2013)
The best proportion needs to be studied for each species.
Seedlings cultivated under B: R = 70: 30 led treatment also provide high fresh quality, the lowest number of aging leaves and the highest percentage of green leaves, 71. 4%.
The fluorescent lamp promotes the highest leaves, thus increasing the fresh weight of the plant.
However, in this treatment, the number of sensory leaves is also higher, accounting for almost half of the total number of leaves.
The most significant event of leaf aging is the absence of chlorophyll and the disassembly of the photosynthetic apparatus, resulting in reduced photosynthetic energy conversion capacity and efficiency (
2009) falquito, Cassol, magales giunier, Oliveira and bagalin.
This fact may reduce the survival rate of plants during domestication.
Seedlings of sugarcane variety RB862552 grow under Red led, with a higher percentage of survival compared to plants grown under fluorescent lamps (
Oliveira Rocha and Scivittaro, 2013).
In the experiment, all the light sources are divided into tillering, and the multiplication rate is between 2. 22 and 3.
It\'s easy to get 33.
In the red palm, more shoots are observed when exposed to more blue LEDs instead of red (Budiarto, 2010)
And in rape, 100% blue (Li et al. , 2013).
As observed in CTC varieties
07, when cultivating sugarcane plants under FL, the yield was the highest.
The effects of led light on the growth, morphology and tillering of several sugarcane varieties were studied (
Marutta, bodinon, Rossi, ambrosano and rodríz, 2013; Rocha et al. , 2013; Silva et al. , 2014)
It is shown that LEDs are an interesting light source for micro-propagation.
However, the results suggest that the role of spectral light quality in controlling in vitro morphological completion has not yet been fully understood and may vary depending on species and/or variety.
Light quality is an important environmental factor affecting the biological synthesis of photosynthetic pigments.
Plants of Sugarcanevar.
RB867515 cultured under WL presented the highest chlorophyll-a content(Figure 2)
It may be due to the spectral profile of white lighting, and similar reports have been made on other sugarcane varieties (Silva et al. , 2014).
In the blue/red LED spectrum, only the processing with a higher proportion of blue LED (B:R=70:30)
Unlike the others, with-a, Chl-b, and Chl (a+b)contents.
This behavior also occurs in FL, indicating that the maximum and minimum light intensity is applied (
B: R = 70: 30 and FL, respectively)
Did not benefit the synthetic pigment.
Highest chlorophyll
A content in sugarcane varieties CTC-
07 production under 100% blue led lights (Maluta et al. , 2013).
However, the chlorophyll content of test-tube seedlings grown under different light qualities may be related to species or varieties (Li et al. , 2013).
Carotene is an auxiliary pigment of the mitochondrial antenna complex.
They might be able to help in the photo.
The protection of the Chlor molecule, determining the consumption of excess energy and the protection of toxic reactive oxygen species (
Milonudama and harvo, 2013).
In the current study, the content of sugarcane leaf carrot under White and B: R LED treatment was higher than that of fluorescence.
In fact, it is one of the advantages of LED spectrum.
Conclusion This study shows that LED-
Basic light has potential advantages for in vitro culture of sugarcane varieties. RB867515.
The spectral quality provided by blue/red LEDs combined in equal proportions is an effective light source for plant development.
In addition, the physiological quality of sugarcane plants was improved.
Current results should be confirmed by analysis with other sugarcane varieties. Doi: 10.
4025/actascibilsci. v38i2.
31109 confirm the financial support of CNPq for this study (
National Center for Natural Science and Technology).
The author hopes to thank the National Institute of Science and Technology (Infos-
National Institute of oceans, optoelectronic-
Eletronica e Spintronica)
Miguel Lales, governor of the biological factory (
Whale wax, Recife (PE)
Used to provide sugar cane.
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February 25, 2016 received.
Accepted on April 24, 2016.
Marina Mediros, arojo Silva (1)
*, Liberal Barbozade Oliveira (2)
Alevira-RonaldoFilho (2)
Trey Tianjin. In Kamala (2)
Lily Willadino (2)
And Atur guvascia. Neto (2)(1)
National University of America, Av.
Francisco Lopez deAlmeida, s/n, 58434-
700, Campina Grande, palaíba, Brazil. (2)
Federal country university of bonambuco, Recife, bonambuco, Brazil.
* Author of communication. E-
Postage: marinamederosas @ yahoo. com.
Br description: Figure 1.
Spectral distribution of relative energy of LEDs and fluorescent lamps.
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